antibacterial effect of silver nanoparticles against four foodborne pathogens

conclusions: silver nanoparticles showed great antibacterial effectiveness on four important foodborne pathogens. therefore, ag nps could be a good alternative for cleaning and disinfection of equipment and surfaces in food-related environments. results: the mic values of ag nps against tested pathogens were in the range of 3.12-6.25 µg/ml. while listeria monocytogenes showed the mic value of 6.25 µg/ml, escherichia coli o157:h7, salmonella typhimurium and vibrio parahaemolyticus all showed the mic values of 3.12 µg/ml. however, all the pathogens showed the same mbc value of 6.25 µg/ml. to obtain an efficient bactericidal activity against e. coli o157:h7 and s. typhimurium, the exposure time should be at least ca. 6 hours., while this time was ca. 5 hours for v. parahaemolyticus and ca. 7 hours for l. monocytogenes. objectives: antimicrobial effect of a commercial nanosilver product, nanocid® l2000, against some foodborne pathogens was evaluated. materials and methods: minimum inhibitory concentrations (mic) were determined by monitoring the growth of bacteria at 600 nm, after 24 hours incubation at 35°c. minimum bactericidal concentrations (mbc) were determined based on 3 log decrease in the viable population of the pathogens after incubation of nutrient agar plates at 35°c for 24 hours. the required exposure time for 3 log reduction in the viable population of the tested pathogens was determined as the minimum exposure time for efficient bactericidal activity. background: there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect.